- Home
- Search Results
- Page 1 of 1
Search for: All records
-
Total Resources4
- Resource Type
-
00040
- Availability
-
40
- Author / Contributor
- Filter by Author / Creator
-
-
Li, Hongquan (4)
-
Prakash, Manu (2)
-
Benoit du Rey, François (1)
-
Bhalla, Amneet Pal (1)
-
Cambournac, Pierre (1)
-
Chai, Chew (1)
-
Drees, Leonard (1)
-
Ellington, Clayton (1)
-
Fire, Andrew Z. (1)
-
Griffith, Boyce E. (1)
-
Hall, Richard Nelson (1)
-
Hol, Felix J. H. (1)
-
Khariton, Margarita (1)
-
Krishnamurthy, Deepak (1)
-
Kumar, Shailabh (1)
-
Larson, Adam G. (1)
-
Leal-Ortiz, Sergio (1)
-
Li, Ethan (1)
-
Liang, Dongfang (1)
-
Lin, Zhonglu (1)
-
- Filter by Editor
-
-
& Spizer, S. M. (0)
-
& . Spizer, S. (0)
-
& Ahn, J. (0)
-
& Bateiha, S. (0)
-
& Bosch, N. (0)
-
& Brennan K. (0)
-
& Brennan, K. (0)
-
& Chen, B. (0)
-
& Chen, Bodong (0)
-
& Drown, S. (0)
-
& Ferretti, F. (0)
-
& Higgins, A. (0)
-
& J. Peters (0)
-
& Kali, Y. (0)
-
& Ruiz-Arias, P.M. (0)
-
& S. Spitzer (0)
-
& Spitzer, S. (0)
-
& Spitzer, S.M. (0)
-
(submitted - in Review for IEEE ICASSP-2024) (0)
-
- (0)
-
-
Have feedback or suggestions for a way to improve these results?
!
Note: When clicking on a Digital Object Identifier (DOI) number, you will be taken to an external site maintained by the publisher.
Some full text articles may not yet be available without a charge during the embargo (administrative interval).
What is a DOI Number?
Some links on this page may take you to non-federal websites. Their policies may differ from this site.
-
Hall, Richard Nelson ; Weill, Uri ; Drees, Leonard ; Leal-Ortiz, Sergio ; Li, Hongquan ; Khariton, Margarita ; Chai, Chew ; Xue, Yuan ; Rosental, Benyamin ; Quake, Stephen R. ; et al ( , Cell Reports Methods)
-
Kumar, Shailabh ; Hol, Felix J. H. ; Pujhari, Sujit ; Ellington, Clayton ; Narayanan, Haripriya Vaidehi ; Li, Hongquan ; Rasgon, Jason L. ; Prakash, Manu ( , Nature Communications)
Abstract Mosquito bites transmit a number of pathogens via salivary droplets deposited during blood-feeding, resulting in potentially fatal diseases. Little is known about the genomic content of these nanodroplets, including the transmission dynamics of live pathogens. Here we introduce Vectorchip, a low-cost, scalable microfluidic platform enabling high-throughput molecular interrogation of individual mosquito bites. We introduce an ultra-thin PDMS membrane which acts as a biting interface to arrays of micro-wells. Freely-behaving mosquitoes deposit saliva droplets by biting into these micro-wells. By modulating membrane thickness, we observe species-dependent differences in mosquito biting capacity, utilizable for selective sample collection. We demonstrate RT-PCR and focus-forming assays on-chip to detect mosquito DNA, Zika virus RNA, as well as quantify infectious Mayaro virus particles transmitted from single mosquito bites. The Vectorchip presents a promising approach for single-bite-resolution laboratory and field characterization of vector-pathogen communities, and could serve as a powerful early warning sentinel for mosquito-borne diseases.
-
Krishnamurthy, Deepak ; Li, Hongquan ; Benoit du Rey, François ; Cambournac, Pierre ; Larson, Adam G. ; Li, Ethan ; Prakash, Manu ( , Nature Methods)